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Induced Spawning of FISHES

Ovaprim is a liquid peptide supplement that is used:

  • to compress the spawning season,
  • to coordinate spawning times,
  • to increase milt production in males,
  • safely and with predictable result

Ovaprim utilizes the fish’s own hormonal control mechanism to safely induce maturation and coordinate spawning dates. When used the normal spawning cycle, Ovaprim can synchronize and coordinate maturation in treated fish by significantly advancing maturation without affecting viability or fecundity. Ovaprim has been tested and proven effective in 6 species of salmonids and several other cultured species. Ovaprim is a licensed and registered product throughout the universe.

Ovaprim

  • contains an analogue of salmon GnRH, the native peptide found in most teleost fish,
  • also contains a dopamine inhibitor that is required in many cultured fish species,
  • is a peptide supplement that is delivered in an inert vehicle,
  • comes in a formulated concentration that can be used in any size of fish,
  • will begin to induce maturation immediately (species dependant) after injection for fast results.

A single dose of Ovaprim is normally sufficient to induce maturation. Initial priming doses can be used to potentiate maturational effects. Ovaprim comes in self-sealing bottles of two sizes, 10 and 100ml. Injections of Ovaprim are delivered to the peritoneal (abdominal) cavity using a standard needle and syringe. Ovaprim can be used in photoperiod-controlled or temperature-controlled fish and can be used in conjunction with other therapeutants as advised by a veterinarian.

Ovaprim has greatest efficacy when used:

  • on fish with a documented history of spawning dates,
  • to synchronize and coordinate maturation during the normal spawning season,
  • to advance spawning times of later spawning fish in the normal spawning season,
  • to increase milt production in males.

In natural spawning, nerve cells in the brain deliver GnRH to the pituitary. The GnRH serves to liberate gonadotropins from pituitary cells. The gonadotropins then direct maturation of the gonads through gonadal steroid hormones. Ovaprim contains an analogue of salmon GnRH and a brain neurotransmitter (dopamine) inhibitor. The GnRH in Ovaprim elicits the release of stored gonadotropins from the pituitary. The dopamine inhibitor serves to remove any other inhibition of GnRH release and effect. The outcome of using Ovaprim is a burst of ovulatory hormones from the pituitary that induces final maturation of the gametes via gonadal steroid hormones. These steroid hormones are essential to final gamete maturation. The final maturation of gametes using Ovaprim does not interfere with spawning behaviour or gamete viability.

Ovaprim can expedite the maturation process in a predictable and repeatable fashion. Ovaprim supplies an exogenous source of salmon GnRH analogue which is more potent than either native salmon GnRH or LHRH. When used during the spawning season, the salmon GnRH analogue in Ovaprim immediately promotes the release of stored gonadotropins from the pituitary. This action of using the fishes own endocrine system works without adversely affecting viability or fecundity.

Ovaprim has the following uses:

  • Induces maturation before the normal spawning season:
  • stimulates milt production for a longer period and for greater volume,
  • moves fish forward in the spawning season,
  • maximizes reproductive potential in all spawners within a population,
  • conserves genetic material in a population, between populations or in endangered stocks.
  • Coordinates maturation during the normal spawning season:
  • spawns a population in a shorter period of time,
  • maximizes hatchery labour and infrastructure by concentrating efforts,
  • moves fish through lifestages as a tightly-packed group according to spawning date,
  • reduces overlap of hatchery duties due to protracted development of hatchery fish.
  • Re-starts stalled maturation:
  • handling stress may arrest maturation, Ovaprim can restart the process,
  • reversing the affects of handling is done by the same action of stimulating the fishes own endocrine system,
  • using Ovaprim post-transport may reduce the incidence of transport shock.
     

How to Use:

Anesthetize the fish as directed by a veterinarian using the appropriate dose of tricane methanesulphonate (TMS) or Marinil. Once anesthetized, weigh the fish and return to the anesthetic bath. Determine the dose for that fish. If the population has a small variance in median weight, a single dose of Ovaprim can be used for all fish that negates weighing. Work with sterile equipment and clean surfaces to limit infection potential.

For use in salmonids: invert the fish and hold head down in the anesthetic bath. This reduces the pressure on the fishes gills caused by the weight of internal organs as you invert and suspend the fish. Under the fishes left pelvic fin is a depression that has few or no scales. This area also has a thin body wall. At this location penetrate the body wall and slowly deliver the Ovaprim into the body cavity. The suspended fish will have its gonads at the forward portion of the body cavity and the left gonad is generally smaller due to the positioning of the liver. Observe if there is any bulging of adjacent tissues; this indicates an intramuscular or subcutaneous injection and reinsertion of the needle will be required. Before withdrawing the needle, leave it in place for a few seconds after delivery of the Ovaprim; this will prevent back-seepage. Transfer the fish to a clean aerated bath to recover.

For other, smaller species: secure fish on a soft wet surface such as a sponge. The heat from your hand may harm some smaller, coldwater fish; handling for extended periods should be avoided. Place the fish on its side and inject into the rear abdominal cavity to avoid contact with internal organs. Smaller fish may require and appropriate needle size that will take longer to deliver the liquid. As with salmonids, leave the needle in place for a moment after delivery to prevent seepage. Transfer the fish to a clean aerated bath to recover.

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How to Spawn....
Pelmatochromis kribensis Boulenger
Kribensis or colloquial deep person frequenting to name it with Krisbensis constitutes one of fish cichlids that kin indigenous Cichlid.
Embryotic origin of this cichlids' fish is indigenous African continent, correct it at along Kribi's river, austral of Cameroon State, West sectioned Africa. Are not odd this fish is denominated according to place where it originates.
Measure of cichlids’ type fish this is not oversized, namely ranging among 3 inches / 7.5 cm, and beginning gets mature on size 2 inches / 5 cm.
Indigenous fish African it has temperament that mousy, so needed by shelter / shelter and also rocks what does take after cave.
Needed temperature ranging 23 – 30 O  C.
Good sexed distinction male and also female gets to be seen of distinctive measure, color and also form, it can be differentiated by easy for civilian even if. On kribensis is measure red blooded it is even greater than kribensis is female at the age same, and its even form is even greater. On kribensis female forms its belly more rounded, and its more yellowish color. On kribensis its behavioral female unique few which is with the number of times curves bend body by issues purplish color on its belly
 
 
 
Spawning KRIBENSIS  (commerce language at Indonesian: Krisbensis)

kribensiskribensis couple

Krisbensis is one of gender ornament fish cichlids (cichlids) one that indigenous West Africa, which is at river waters. It is amends flourishes with spawn, where does this egg hide in one place which takes after cave.
 
How to Spawn
Spawning Kribensis is regarded some factor, amongst those:
 
Outer factor (environmentally)  :
Total Kribensis's tide: 1 tide
Genital determination: manly longer, having black circle on dorsal's fins. Meanwhile female has belly that rounded, its body is shorter of male. Ordinary sold more female than red blooded. Resulting sex ratio clings to PH, if PH high(>7, alkali) result more a lot of masculine, meanwhile PH contemns(< 7, acid) result more a lot of female. PH = 7 (normal) male ratio and female is proportional
tank measure: 60 x 30 x 30 cm
With aquarium is battened down.
After water with longing one needs it, and filter is corner.
Sand that is utilized: sand whatever would be convenient for growth is good. Better dashed with by stone – largish rock (jumbled). One that needs to be reassured is sand thickness that adequately, more or less as thick as 3 cm.
Living plant: kribensis is nuts over green plant, adequately two plant pots in this case. Suggested to utilize plant that soars tall. Water plant a sort Cabomba, Myriopillium and also Hydrilla can be utilized.
Other fish: in function as dither fish / target fish one that makes kribensis enough attractive since its few mousy character.
In the presence this fish important enough that calls away the attention to fish out masculine and also female for joins together mutually.  Fish that I utilizes: 1 number Corydoras albino  (outgrow), 1 number Bronze corydoras (little), 1 Rainbow Peacock's tide (be), 1 number Celebese rainbow  (female, insufficiently attractive), and 1 Rasbora's tide. 
Substrate who is utilized for spawn: –cave as PVC ½ circle, long 15 cm.
 
Internal factor
Kribensis's couple has age enough (approximately 8 months). Food has always available, utilize waters bug living weft which a lot of, little bit ground worm, and ices worm knockabout food. –heart in alimentary application. Stick out for water condition that do ever in a state clear.
 
Feed back  : don't utilize coral’s rock or rock whatever that contain calcium element, don't available Kribensis another one, so enough 1 tide, aquarium shall ever give cover to prevent input and growing dust its algae, ever be utilize dither fish, utilize green plant that adequately, provide cave.
 
After successful spawned, constraint that have once my natural is magnified problem, where is amount resulted quite a lot (60) number so endeavors that fish is not stress. That trick fish is not stress: total aquaria deep fish not heaving full, given by water plant, and water situation always clear.
 
Amplification
Glued egg on substrate will hatch in duration 4 days. Egg will get to find at the bottom with egg yolk that stills to stick on spawn’s body.

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